Learn about cystic fibrosis, a genetic disorder that affects the lungs, pancreas, and other organs, and how to treat and live with this chronic disease.
CF is a rare genetic disease found in about 30,000 people in the U.S. If you have CF or are considering testing for it, knowing about the role of genetics in CF can help you make informed decisions about your health care.
If you or your child has just been diagnosed with cystic fibrosis, or your doctor has recommended testing for CF, you may have many questions.
Diagnosing CF is a multistep process. A complete diagnostic evaluation should include a newborn screening, a sweat chloride test, a genetic or carrier test, and a clinical evaluation at a CF Foundation-accredited care center.
Raising a child with cystic fibrosis can bring up many questions because CF affects many aspects of your child’s life. Here you’ll find resources to help you manage your child’s daily needs and find the best possible CF care.
Living with cystic fibrosis comes with many challenges, including medical, social, and financial. By learning more about how you can manage your disease every day, you can ultimately help find a balance between your busy lifestyle and your CF care.
People with CF are living longer, healthier lives than ever before. As an adult with CF, you may reach key milestones you might not have considered. Planning for these life events requires careful thought as you make decisions that may impact your life.
People with cystic fibrosis are living longer and more fulfilling lives, thanks in part to specialized CF care and a range of treatment options.
Cystic Fibrosis Foundation-accredited care centers provide expert care and specialized disease management to people living with cystic fibrosis.
We provide funding for and accredit more than 120 care centers and 53 affiliate programs nationwide. The high quality of specialized care available throughout the care center network has led to the improved length and quality of life for people with CF.
The Cystic Fibrosis Foundation provides standard care guidelines based on the latest research, medical evidence, and consultation with experts on best practices.
As a clinician, you’re critical in helping people with CF maintain their quality of life. We’re committed to helping you partner with patients and their families by providing resources you can use to improve and continue to provide high-quality care.
As part of the Cystic Fibrosis Foundation's mission to help improve the lives of people living with cystic fibrosis, the PSDC initiative taps the CF community to inform key efforts to support the management of daily care.
Your cystic fibrosis care team includes a group of CF health care professionals who partner with you to provide specialized, comprehensive CF care.
Many people living with cystic fibrosis and their families face complicated issues related to getting the care they need. Cystic Fibrosis Foundation Compass makes sure that no one has to do it alone.
CF Foundation Compass is a service that helps people with CF and their families with navigating insurance options, connecting to legal information and experts, finding available financial resources, and tackling other life issues.
CF care team members are paramount in providing highly specialized care to people living with CF. CF Foundation Compass can help by serving as a strategic ally for care teams, so team members can focus on their patients’ care.
CF Foundation Compass can help you navigate insurance, financial, legal, and other issues you are facing. Use this online form to start your conversation with a Compass case manager today.
The Cystic Fibrosis Foundation is the world’s leader in the search for a cure for CF and supports a broad range of research initiatives to tackle the disease from all angles.
The CF Foundation offers a number of resources for learning about clinical trials and treatments that are being developed to improve the treatment of cystic fibrosis.
Our understanding of CF continues to evolve as scientists study what causes the disease and how it affects the body. These insights drive the development of new and better treatments and bring us one step closer to a cure.
Researchers, supported by the CF Foundation, have made tremendous advances to improve the health and quality of life of people with CF. We are committed to providing the tools and resources you need to continuously build upon this work.
Knowledge of cystic fibrosis transmembrane conductance regulator (CFTR) protein structure will contribute toward the understanding of CFTR function and CF biology, the mechanisms of action for CF drugs, and provide additional insight toward new drug discovery through structure-based drug design.
Accelagen provides comprehensive protein purification services for drug discovery and pre-clinical studies. Accelagen's protein purification expertise includes purification of full-length CFTR and many nucleotide binding domain (NBD) constructs of CFTR.
With funding and guidance from Cystic Fibrosis Foundation Therapeutics Inc. (CFFT), Accelagen has established in-house systems that enable them to express and purify full-length CFTR. With technical guidance by Dr. Jack Riordan and his colleaguesfrom the University of North Carolina at Chapel Hill, Accelagen is able to generate enough biomass essential for purification of CFTR at milligram scale, using Cell Factories to produce in large-scale stably transfected BHK cells.
Recombinant NBDs can also be made for functional and structural studies. With technical guidance by former SGX Pharmaceutical scientists, Accelagen has successfully purified the following NBD1 and NBD2 domains of CFTR:
Certain NBD domain proteins, e.g., 5762c54BSt1pi1, are only expressed in soluble form at a significant level in the presence of chaperone co-expression, hence the above plasmid for co-expression, or competent cells harboring the chaperone protein, are generated and available to researchers.
Accelagen can provide protein expression in mammalian, baculovirus, and E. coli expression systems. Accelagen has protein purification capabilities with extensive selections of chromatography media and flexible configuration of chromatography columns tailored to each protein.
Please contact Accelagen directly regarding more detail about CFTR protein expression and purification capabilities on a fee-for-service basis.
6044 Cornerstone Ct W, Ste. C
San Diego, CA 92121
Fax: 888-239-6490Toll-free: 888-365-8085
Dr. Christie Brouillette is funded by CFFT to provide limited amounts of purified NBDs to the CF research community for pilot functional and structural studies. Typically, sub-milligram to several milligrams of pure NBDs can be provided frozen in a standard buffer containing adenosine triphosphate (ATP).
Proteins are expressed in an E. coli expression system according to the methods found in Atwell et al. (2010) PEDS 23:375-384 and Lewis et al., (2005) JBC 280:1346-1353. Plasmids for expression have been obtained from DNASU Plasmid Repository at the Arizona State University Biodesign Institute.
The following human NBD proteins are currently available. New proteins are continually added to the list so check for availability. Please contact Christie Brouillette at email@example.com for additional information to request protein.
Additional protein constructs of NBD1 are available through the CFTR Folding Consortium.
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